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check_url/65246?article_type=b
Direct Synthesis of Gold Nanoparticles for Protein Localization Analysis
April 28, 2023
check_url/65246?article_type=b
細胞内EM可視金ナノ粒子の直接合成によるタンパク質局在解析(英語)
01:42 min
•
check_url/65246?article_type=b
細胞培養用のサファイアディスクの調製
01:23 min
•
check_url/65246?article_type=b
サファイアディスクキャリアアセンブリの高圧凍結(HPF)、凍結置換固定(FSF)および再水和
03:20 min
•
check_url/65246?article_type=b
サファイアディスクを用いた哺乳類細胞の金ナノ粒子合成
03:23 min
•
Zhaodi Jiang
2
1
National Institute of Biological Sciences, Beijing
,
2
Tsinghua Institute of Multidisciplinary Biomedical Research
,
Tsinghua University
Summary
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本プロトコルは、新規自己核生成抑制機構に基づく金ナノ粒子合成技術を用いて細胞内のメタロチオネインタグ付きタンパク質を検出するためのクローン可能な電子顕微鏡標識技術を記載する。
Article
