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Direct Synthesis of EM-Visible Gold Nanoparticles in Cells for Protein Localization Analysis with Well-Preserved Ultrastructure (Video) | JoVE
Preparing Sapphire Discs for Cell Culture (Video) | JoVE
High-Pressure Freezing (HPF), Freeze-Substitution Fixation (FSF) and Rehydration of Sapphire Disc-Carrier Assembly (Video) | JoVE
Gold Nanoparticle Synthesis for Mammalian Cells Using Sapphire Discs (Video) | JoVE
本プロトコルは、新規自己核生成抑制機構に基づく金ナノ粒子合成技術を用いて細胞内のメタロチオネインタグ付きタンパク質を検出するためのクローン可能な電子顕微鏡標識技術を記載する。
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