Direct Synthesis of Gold Nanoparticles for Protein Localization Analysis

April 28th, 2023

Direct Synthesis of EM-Visible Gold Nanoparticles in Cells for Protein Localization Analysis with Well-Preserved Ultrastructure (Video) | JoVE

01:42 min

•

ï»¿Preparing Sapphire Discs for Cell Culture (Video) | JoVE

01:23 min

•

High-Pressure Freezing (HPF), Freeze-Substitution Fixation (FSF) and Rehydration of Sapphire Disc-Carrier Assembly (Video) | JoVE

03:20 min

•

Gold Nanoparticle Synthesis for Mammalian Cells Using Sapphire Discs (Video) | JoVE

03:23 min

•

Zhaodi Jiang^1,2

¹National Institute of Biological Sciences, Beijing, ²Tsinghua Institute of Multidisciplinary Biomedical Research, Tsinghua University

Summary
Automatic Translation

English (Original)
العربية (Arabic)
中文 (Chinese)
dansk (Danish)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
हिंदी (Hindi)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
norsk (Norwegian)
português (Portugese)
русский (Russian)
español (Spanish)
svenska (Swedish)
Türkçe (Turkish)

Mevcut protokol, yeni bir otonükleasyon bastırma mekanizmasına dayalı altın nanopartikül sentez tekniği kullanarak hücrelerdeki metallothionein etiketli proteinleri tespit etmek için klonlanabilir bir elektron mikroskobu etiketleme teknolojisini tanımlamaktadır.

Article

Direct Synthesis of Gold Nanoparticles for Protein Localization Analysis

Get cutting-edge science videos from JoVE sent straight to your inbox every month.