Enhancing Targeted Cancer Diagnosis by Quantifying Single-Cell Mechanics

May 19th, 2023

Shear Assay Protocol for the Determination of Single-Cell Material Properties (Video) | JoVE

02:25 min

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Microfluidic Shear Apparatus Setup for Single-Cell Fluid Shear Assay (Video) | JoVE

02:16 min

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Performing Single-Cell Shear Assay and Imaging (Video) | JoVE

01:51 min

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Processing the Single-Cell Shear Assay Imaging Data (Video) | JoVE

02:11 min

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Luke J. Holen*¹, Killian Onwudiwe*¹, Julian Najera¹, Maksym Zarodniuk¹, John D. Obayemi², Winston O. Soboyejo², Meenal Datta¹

¹Department of Aerospace and Mechanical Engineering, University of Notre Dame, ²Departments of Mechanical and Biomedical Engineering, Worcester Polytechnic Institute

* These authors contributed equally

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Este protocolo describe la cuantificación de las propiedades mecánicas de líneas celulares cancerosas y no cancerosas in vitro. Las diferencias conservadas en la mecánica de las células cancerosas y normales pueden actuar como un biomarcador que puede tener implicaciones en el pronóstico y el diagnóstico.

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Enhancing Targeted Cancer Diagnosis by Quantifying Single-Cell Mechanics

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