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Simple Establishment of a Vascularized Osteogenic Bone Marrow Niche Using Pre-Cast Poly(Ethylene Glycol) (PEG) Hydrogels in an Imaging Microplate (Video) | JoVE
Establishing the 3D Stromal Monoculture (Video) | JoVE
Establishing the 3D Stromal-Endothelial Cell Co-Culture (Video) | JoVE
Quantifying the Endothelial Cell Network Formation After the 3D Stromal-Endothelial Cell Co-Culture (Video) | JoVE
Assessing the Osteogenic Differentiation of 3D Stromal-Endothelial Cells by Monitoring the Alkaline Phosphatase Activity (Video) | JoVE
通过将间充质细胞和内皮细胞接种到预制的3D PEG水凝胶上,建立了骨髓血管壁龛的 体外 模型。壁龛的内皮网络、ECM 成分和 ALP 活性因所使用的生长因子而异。该平台可用于高级癌症模型。
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