Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove

Efficient In Vitro Cultivation of Mouse Cochlear Hair Cells

September 15th, 2023
*1, *2, 2, 2, 2, 2
1Department of Core Research Laboratory, The Second Affiliated Hospital of Xi’an Jiaotong University, 2Department of Otolaryngology, The Second Affiliated Hospital of Xi’an Jiaotong University
* These authors contributed equally

Summary
Automatic Translation

Herein, we present a detailed protocol for isolating and culturing primary cochlear hair cells from mice. Initially, the organ of Corti was dissected from neonatal (aged 3-5 days) murine cochleae under a microscope. Subsequently, cells were enzymatically digested into a single-cell suspension and identified using immunofluorescence after several days in culture.

Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter