Preparation of Electron Microscopy Grids for In Situ Cellular Cryotomography

December 15th, 2023

Optimizing Grid Preparation for Enhanced Cryoelectron Tomography (Video) | JoVE

01:19 min

•

Preparing Electron Microscopy (EM) Grids for Cellular Cryotomography (Video) | JoVE

02:29 min

•

Cell Seeding and Transfection on Electron Microscopy (EM) Grids for Cryotomography (Video) | JoVE

02:04 min

•

Plunge Freezing of Electron Microscopy (EM) Grids for Cryotomography (Video) | JoVE

02:45 min

•

Noah Weber*^1,2, Brennan Hinks*^2,3, Jacob Jensen², Thomas Lidahl^2,3, Luiza Mendonça²

¹College of Liberal Arts, University of Minnesota, ²Department of Biochemistry, Molecular Biology and Biophysics, Medical School, University of Minnesota, ³College of Biological Sciences, University of Minnesota

* These authors contributed equally

Summary
Automatic Translation

English (Original)
العربية (Arabic)
中文 (Chinese)
dansk (Danish)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
हिंदी (Hindi)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
norsk (Norwegian)
português (Portugese)
русский (Russian)
español (Spanish)
svenska (Swedish)
Türkçe (Turkish)

Этот метод обеспечивает доступный и гибкий протокол подготовки сеток электронной микроскопии (ЭМ) для клеточной криотомографии in situ и коррелятивной световой и электронной микроскопии (КЛЭМ).

Article

Preparation of Electron Microscopy Grids for In Situ Cellular Cryotomography

Get cutting-edge science videos from JoVE sent straight to your inbox every month.