Using Induced Pluripotent Stem Cell Suspension for Kidney Organoid Production

September 1st, 2023

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Langping Gao*^1,2, Yue Wang*³, Gang Wang⁴, Hangdi Wu², Qingtao Yan¹, Jingjing Wang¹, Fei Liu¹, Haidong Fu¹, Wei Li⁵, Lidan Hu¹, Jianhua Mao^1,2

¹Department of Nephrology, The Children's Hospital, Zhejiang University School of Medicine, ²Zhejiang University School of Medicine, ³Hubei Normal University, ⁴National Clinical Research Center of Kidney Diseases, Jinling Hospital, Nanjing University School of Medicine, ⁵Clinical Laboratory, The Children’s Hospital, Zhejiang University School of Medicine, National Clinical Research Center for Child Health

* These authors contributed equally

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يقدم هذا البروتوكول طريقة شاملة وفعالة لإنتاج عضويات الكلى من الخلايا الجذعية المستحثة متعددة القدرات (iPSCs) باستخدام ظروف ثقافة التعليق. يكمن التركيز الأساسي لهذه الدراسة في تحديد كثافة الخلايا الأولية وتركيز ناهض WNT ، مما يفيد الباحثين المهتمين بأبحاث الكلى العضوية.

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Using Induced Pluripotent Stem Cell Suspension for Kidney Organoid Production

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