Using Induced Pluripotent Stem Cell Suspension for Kidney Organoid Production

September 1st, 2023

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Langping Gao*^1,2, Yue Wang*³, Gang Wang⁴, Hangdi Wu², Qingtao Yan¹, Jingjing Wang¹, Fei Liu¹, Haidong Fu¹, Wei Li⁵, Lidan Hu¹, Jianhua Mao^1,2

¹Department of Nephrology, The Children's Hospital, Zhejiang University School of Medicine, ²Zhejiang University School of Medicine, ³Hubei Normal University, ⁴National Clinical Research Center of Kidney Diseases, Jinling Hospital, Nanjing University School of Medicine, ⁵Clinical Laboratory, The Children’s Hospital, Zhejiang University School of Medicine, National Clinical Research Center for Child Health

* These authors contributed equally

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이 프로토콜은 현탁 배양 조건을 사용하여 유도만능줄기세포(iPSC)에서 신장 오가노이드를 생산하기 위한 포괄적이고 효율적인 방법을 제시합니다. 이 연구의 주요 초점은 초기 세포 밀도와 WNT 작용제 농도의 결정에 있어 신장 오가노이드 연구에 관심이 있는 연구자에게 도움이 됩니다.

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Using Induced Pluripotent Stem Cell Suspension for Kidney Organoid Production

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