Using Induced Pluripotent Stem Cell Suspension for Kidney Organoid Production

September 1st, 2023

Optimizing iPSC Differentiation for Efficient Production to Generate Kidney Organoids (Video) | JoVE

02:40 min

•

IPSC Differentiation into Posterior Primitive Streak and Nephrogenic Intermediate Mesoderm (Video) | JoVE

02:23 min

•

Generating Kidney Organoids from Induced Pluripotent Stem Cell Suspension Culture (Video) | JoVE

02:36 min

•

Langping Gao*^1,2, Yue Wang*³, Gang Wang⁴, Hangdi Wu², Qingtao Yan¹, Jingjing Wang¹, Fei Liu¹, Haidong Fu¹, Wei Li⁵, Lidan Hu¹, Jianhua Mao^1,2

¹Department of Nephrology, The Children's Hospital, Zhejiang University School of Medicine, ²Zhejiang University School of Medicine, ³Hubei Normal University, ⁴National Clinical Research Center of Kidney Diseases, Jinling Hospital, Nanjing University School of Medicine, ⁵Clinical Laboratory, The Children’s Hospital, Zhejiang University School of Medicine, National Clinical Research Center for Child Health

* These authors contributed equally

Summary
Automatic Translation

English (Original)
العربية (Arabic)
中文 (Chinese)
dansk (Danish)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
हिंदी (Hindi)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
norsk (Norwegian)
português (Portugese)
русский (Russian)
español (Spanish)
svenska (Swedish)
Türkçe (Turkish)

Bu protokol, süspansiyon kültürü koşulları kullanılarak indüklenmiş pluripotent kök hücrelerden (iPSC'ler) böbrek organoidleri üretmek için kapsamlı ve etkili bir yöntem sunar. Bu çalışmanın birincil vurgusu, başlangıç hücre yoğunluğunun ve WNT agonist konsantrasyonunun belirlenmesinde yatmaktadır, böylece böbrek organoid araştırmalarıyla ilgilenen araştırmacılara fayda sağlamaktadır.

Article

Using Induced Pluripotent Stem Cell Suspension for Kidney Organoid Production

Get cutting-edge science videos from JoVE sent straight to your inbox every month.