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Using Induced Pluripotent Stem Cell Suspension for Kidney Organoid Production

September 1st, 2023
*1,2, *3, 4, 2, 1, 1, 1, 1, 5, 1, 1,2
1Department of Nephrology, The Children's Hospital, Zhejiang University School of Medicine, 2Zhejiang University School of Medicine, 3Hubei Normal University, 4National Clinical Research Center of Kidney Diseases, Jinling Hospital, Nanjing University School of Medicine, 5Clinical Laboratory, The Children’s Hospital, Zhejiang University School of Medicine, National Clinical Research Center for Child Health
* These authors contributed equally

Summary
Automatic Translation

Denne protokollen presenterer en omfattende og effektiv metode for å produsere nyreorganoider fra induserte pluripotente stamceller (iPSCs) ved bruk av suspensjonskulturbetingelser. Hovedvekten av denne studien ligger i bestemmelsen av den opprinnelige celletettheten og WNT-agonistkonsentrasjonen, og derved til fordel for forskere som er interessert i nyreorganoidforskning.

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