Fluorescence-Activated Single-Cell Sorting of Human Mesenchymal Stem Cells

November 10th, 2023

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Ayona Gupta*¹, Risani Mukhopadhyay*¹, Himanshi Khandelwal¹, Narendra Nala², Uttara Chakraborty¹

¹Manipal Institute of Regenerative Medicine, Bengaluru, Manipal Academy of Higher Education, Manipal, ²HIV-AIDS Laboratory, Molecular Biology and Genetics Unit, Jawaharlal Nehru Centre for Advanced Scientific Research (JNCASR)

* These authors contributed equally

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Denne protokollen beskriver bruken av fluorescensaktivert cellesortering av humane mesenkymale stamceller ved bruk av enkeltcellesorteringsmetoden. Spesielt kan bruk av enkeltcellesortering oppnå 99% renhet av immunfenotypede celler fra en heterogen populasjon kombinert med en multiparametrisk flowcytometribasert tilnærming.

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Fluorescence-Activated Single-Cell Sorting of Human Mesenchymal Stem Cells

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