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Studying Bipolar and Horizontal Neuronal Cells in the Murine Split Retina

January 16th, 2024

Using the Split Retina Technique for Enhanced Access and Accelerated Experiments (Video) | JoVE

Using the Split Retina Technique for Enhanced Access and Accelerated Experiments (Video) | JoVE

01:44 min

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Mouse Retinal Dissection for Split Retina Application (Video) | JoVE

Mouse Retinal Dissection for Split Retina Application (Video) | JoVE

02:00 min

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Splitting of Murine Retina for Immunofluorescence Analysis (Video) | JoVE

Splitting of Murine Retina for Immunofluorescence Analysis (Video) | JoVE

01:52 min

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Mouse Split Retina Preparation for Immunofluorescence Analysis (Video) | JoVE

Mouse Split Retina Preparation for Immunofluorescence Analysis (Video) | JoVE

02:51 min

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Ryan M. Hecht¹, Qing Shi¹, Tavita R. Garrett², Benjamin Sivyer², Catherine Morgans¹

¹Department of Chemical Physiology and Biochemistry, Oregon Health and Science University, ²Casey Eye Institute, Oregon Health and Science University

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这项工作提出了一种替代的扁平安装视网膜制剂，其中去除感光细胞体可以更快地传播抗体并改善贴片移液器对视网膜内神经元的访问，以进行免疫组织化学、原位杂交和电生理学实验。

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