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Monitoring Epileptiform Events in Drosophila via Calcium Imaging

October 13th, 2023
1, 2, 2, 2, 3,4, 1
1Department of Neurology, Institute of Neuroscience, Key Laboratory of Neurogenetics and Channelopathies of Guangdong Province and the Ministry of Education of China, the Second Affiliated Hospital, Guangzhou Medical University, 2The Second Clinical Medicine School of Guangzhou Medical University, 3Department of Obstetrics and Gynecology, Center for Reproductive Medicine, Guangdong Provincial Key Laboratory of Major Obstetric Diseases, The Third Affiliated Hospital of Guangzhou Medical University, 4Key Laboratory for Reproductive Medicine of Guangdong Province, The Third Affiliated Hospital of Guangzhou Medical University

Summary
Automatic Translation

ここでは、てんかん様活性をモニターするために、GCaMP6を発現する成虫ショウジョウバエのex vivoカルシウムイメージングのプロトコルを紹介します。プロトコルは細胞レベルでのてんかんの潜在的なメカニズムの調査を可能にする前の生体内カルシウムイメージ投射によって大人のショウジョウバエのictalでき事を調査するための貴重な用具を提供する。

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