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A Bicelle Crystallization Setup for ABC Transporter Membrane Proteins to Advance Drug Development (Video) | JoVE
Reductive Methylation and Pre-Crystallization Treatment of Purified Heterodimeric ABCG5/G8 Protein (Video) | JoVE
Crystallization of Heterodimeric ABCG5/G8 Protein in Lipidic Bicelle Using Hanging Drop Vapor Diffusion (Video) | JoVE
该协议描述了用于结晶甾醇转运蛋白ABCG5 / G8的设置。ABCG5/G8被复构成双细胞进行悬滴结晶。该协议不需要专门的材料或底物,因此可以在任何实验室中轻松使用,以通过X射线晶体学确定蛋白质结构。
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