Extraction and Enzymatic Purification of Microglia from the Mouse Spinal Cord

September 22nd, 2023

Microglia Research on Spinal Cord Heterogeneity and Purification (Video) | JoVE

01:52 min

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Liberase-Based Enzymatic Purification of Microglial Cells Isolated from a Mouse Spinal Cord (Video) | JoVE

02:42 min

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Claudia I Gutiérrez-Román^1,2, María E Meléndez Camargo², Cuauhtémoc C García Rojas³, Miguel Jimenez Olvera⁴, Sandra H Gutiérrez Román⁵, Oscar Medina-Contreras¹

¹Epidemiology, Endocrinology & Nutrition, Mexico Children's Hospital, ²Pharmacy Department, National School of Biological Sciences, National Polytechnic Institute, ³Graduate Department, Dr. Eduardo Liceaga General Hospital of Mexico, ⁴Pain Clinic and Palliative Care, Dr. Eduardo Liceaga General Hospital of Mexico, ⁵General Surgery Service, Metropolitan Angeles Hospital

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تعتبر الخلايا الدبقية الصغيرة من أكثر الخلايا تنوعا في الجسم ، وهي قادرة على التكيف المورفولوجي والوظيفي. يتيح عدم تجانسها وتعدد وظائفها الحفاظ على توازن الدماغ ، بينما ترتبط أيضا بأمراض عصبية مختلفة. هنا ، يتم وصف تقنية لتنقية الخلايا الدبقية الصغيرة للحبل الشوكي.

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Extraction and Enzymatic Purification of Microglia from the Mouse Spinal Cord

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