Japan Women's University 2 articles published in JoVE Biology Establishment of a Clonal Culture of Unicellular Conjugating Algae Yuki Tsuchikane1, Takashi Hamaji2, Katsumi Ota3, Syou Kato4 1 In this article, we demonstrate the establishment of clonal cultures of unicellular conjugating algal species collected from a natural field site. Biochemistry Preparation of Giant Vesicles Encapsulating Microspheres by Centrifugation of a Water-in-oil Emulsion Yuno Natsume1, Hsin-i Wen2, Tong Zhu2, Kazumi Itoh1, Li Sheng2, Kensuke Kurihara2,3,4 1 Giant vesicles containing highly packed micrometer-sized components are useful cell models. The water-in-oil emulsion centrifugation method is a simple, powerful tool for the preparation of giant vesicles with encapsulated materials.