Charles River View Institution's Website 8 articles published in JoVE Cancer Research Sectioning Mammary Gland Whole Mounts for Lesion Identification Deirdre K Tucker1,2, Julie F Foley3, Schantel A Bouknight4, Suzanne E Fenton2 1Curriculum in Toxicology, University of North Carolina at Chapel Hill, 2National Toxicology Program Laboratory (NTPL), DNTP, National Institute of Environmental Health Sciences, 3Cellular and Molecular Pathology Branch, DNTP, National Institute of Environmental Health Sciences, 4Charles River Laboratories Inc. We developed a method to successfully remove, process, section, and stain, for histopathological evaluation, mammary tissue that had originally been fixed on slides as whole mounts. This method may promote the collection and evaluation of mammary gland whole mounts in reproductive and developmental test guideline studies. Behavior Nest Building as an Indicator of Health and Welfare in Laboratory Mice Brianna N. Gaskill1, Alicia Z. Karas2, Joseph P. Garner3,4, Kathleen R. Pritchett-Corning1 1Research Models and Services, Charles River, 2Department of Clinical Sciences, Tufts University, 3Department of Comparative Medicine, Stanford University, 4Department of Psychiatry and Behavioral Sciences, Stanford University We demonstrate the utility of nest building behavior in laboratory mice as an indicator of welfare. Nest scoring is a sensitive technique that is altered by temperature, illness, and aggression. The time to integrate into nest test (TINT) is a simple cage-side assessment that can detect postoperative pain. Biology Manual Restraint and Common Compound Administration Routes in Mice and Rats Elton Machholz1, Guy Mulder2, Casimira Ruiz1, Brian F. Corning1, Kathleen R. Pritchett-Corning2 1Insourcing Solutions, Charles River, 2Research Models and Services, Charles River Working safely and humanely with research rodents requires a core competency in handling and restraint methods. This article will present the basic principles required to safely handle and effectively administer compounds to mice and rats. Biology Multiplexed Fluorometric ImmunoAssay Testing Methodology and Troubleshooting Michelle L. Wunderlich1, Megan E. Dodge1, Rajeev K. Dhawan1, William R. Shek1 1Research Animal Diagnostic Services (RADS), Charles River Using Luminex Corporation’s xMAP microsphere technology, we have developed the Multiplexed Fluorometric ImmunoAssay (MFIA) for serosurveillance of various laboratory animal species. The MFIA is a suspension microarray where antigen, tissue control or immunoglobulins are covalently linked to color-coded polystyrene microspheres. The MFIA testing method as well as various troubleshooting topics is addressed. Biology Mouse Sperm Cryopreservation and Recovery using the I·Cryo Kit Ling Liu1, Steven R. Sansing1, Iva S. Morse1, Kathleen R. Pritchett-Corning2 1Genetically Engineered Models and Services, Charles River, 2Research Models and Services, Charles River Here we demonstrate the newly developed I•Cryo kit for mouse sperm cryopreservation. Two-cell stage embryo development with frozen-thawed sperm was improved consistently in 5 mouse strains with the use of this kit. Over a 1.5 year period, 49 genetically modified mouse lines were archived by sperm cryopreservation with the I•Cryo kit and later successfully recovered by IVF. Immunology and Infection Diagnosis of Ecto- and Endoparasites in Laboratory Rats and Mice Christina M. Parkinson1, Alexandra O'Brien1, Theresa M. Albers1, Meredith A. Simon1, Charles B. Clifford1, Kathleen R. Pritchett-Corning2,3 1Research Animal Diagnostic Services, Charles River, 2Research Models and Services, Charles River, 3Department of Comparative Medicine, University of Washington This article describes various procedures for screening rats and mice to detect endo- or ectoparasitism. Several diagnostic assays will be demonstrated, both those suitable for use on live animals and those used after euthanasia of the animal. Photographs to aid in identification of rat and mouse parasites will be included. Biology Diagnostic Necropsy and Selected Tissue and Sample Collection in Rats and Mice Christina M. Parkinson1, Alexandra O'Brien1, Theresa M. Albers1, Meredith A. Simon1, Charles B. Clifford2, Kathleen R. Pritchett-Corning2,3 1Research Animal Diagnostic Services, Charles River, 2Research Models and Services, Charles River, 3Department of Comparative Medicine, University of Washington This article describes the procedures for conducting a basic postmortem examination of a mouse or rat, and the collection of basic organs, as well as more challenging sample types from for histological, microbiological, and PCR evaluation. Biology Principles of Rodent Surgery for the New Surgeon Kathleen R. Pritchett-Corning1, Guy B. Mulder1, Yiying Luo1, William J. White1 1Charles River, Research Models and Services Before attempting surgery, a new surgeon should have training in basic surgical techniques and concepts. This article will present basic surgical considerations with an emphasis on rodents.