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Biology

리트머스 테스트를 사용하여 박테리아의 비색 검출

Published: September 17, 2016 doi: 10.3791/54546

Materials

Name Company Catalog Number Comments
Ethylenediaminetetraacetic acid (EDTA) VWR AMRESCO 0105
Sodium Hydroxide (NaOH) pellets BIO BASIC CANADA INC. SB6789
Tris-base VWR AMRESCO 0497
Boric acid AMRESCO 0588
Urea VWR AMRESCO M123
40% acrylamide/bisacrylamide (29:1) solution BIO BASIC CANADA INC. A0007
Sucrose Bioshop Canada inc. SUC507
Bromophenol blue Bioshop Canada inc. BRO777
Xylenecyanol FF SIGMA-ALDRICH X-4126
10% sodium dodecyl sulfate Bioshop Canada inc. SDS001
Hydrochloric Acid (HCl) CALEDON LABORATORIES LTD 6026
Sodium Chloride (NaCl) Bioshop Canada inc. SOD001
4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) Bioshop Canada inc. HEP001
Magnesium Chloride (II) hexahydrate VWR AMRESCO 0288
Tween 20 Bioshop Canada inc. TW508
Adenosine Triphospahte (ATP) AMRESCO 0220
Sodium Acetate trihydrate (NaOAc) SIGMA-ALDRICH S8625
Ethanol Commercial Alcohols P016EAAN
Tetramethyleneethylenediamine (TEMED) AMRESCO 0761
10% Ammonium persulfate (APS) BIO BASIC CANADA INC. AB0072
Succinimidyl 4-(N-maleimidomethyl) cyclohexane-1-carboxylate (SMCC) ThermoFisher SCIENTIFIC 22360
Dimethyl sulfoxide (DMSO) CALEDON LABORATORIES 803540
Urease SIGMA-ALDRICH U0251
1x Phosphate Buffered Saline (PBS) ThermoFisher SCIENTIFIC 70011-069
0.04% Phenol red SIGMA-ALDRICH P3532
10x T4 polynucleotide kinase reaction buffer Lucigen 30061-1
10x T4 DNA ligase reaction buffer Bio Basics Canada B1122-B
T4 DNA ligase (5 U/μl) Thermo Fischer Scientific B1122
Luria Bertani (LB) Broth AMRESCO J106
Agar AMRESCO J637
T4 polynucleotide kinase (10 U/μl) Lucigen 30061-1
E. coli K12 (MG1655) ATCC ATCC700926
Centrifuge Beckman Coulter, Inc. 392187
Glass plates CBS scientific ngp-250nr
0.75 mm thick spacers CBS scientific VGS-0725r
12-well comb CBS scientific VGC-7512
UV Lamp UVP 95-0017-09
Spectrophotometer (NanoVue) GE Healthcare N/A
Metal plate CBS scientific CPA165-250
Vortex VWR International 58816-123
Gel electrophoresis apparatus CBS scientific ASG-250
Petri dishes VWR International 25384-342
100 kDa MWCO centrifugal filters EMD Millipore UFC510024
Magnetic Bead (BioMag) Bangs Laboratories Inc BM568
Magnetic Seperation Rack New England BioLabs S1506S
Microfuge tubes Sarstedt 72.69
Syringe filter (0.22 μm) VWR International 28145-501
14 ml culture tube VWR International 60818-725
Cell culture incubator Eppendorf Scientific M13520000
Branson Ultrasonic cleaner Branson N/A
Camera (Canon Powershot G11) Canon N/A
50 ml conical tube VWR International 89004-364

DOWNLOAD MATERIALS LIST

References

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리트머스 테스트를 사용하여 박테리아의 비색 검출
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Cite this Article

Tram, K., Manochehry, S., Feng, Q.,More

Tram, K., Manochehry, S., Feng, Q., Chang, D., Salena, B. J., Li, Y. Colorimetric Detection of Bacteria Using Litmus Test. J. Vis. Exp. (115), e54546, doi:10.3791/54546 (2016).

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