Materials
Name | Company | Catalog Number | Comments |
Ethylenediaminetetraacetic acid (EDTA) | VWR AMRESCO | 0105 | |
Sodium Hydroxide (NaOH) pellets | BIO BASIC CANADA INC. | SB6789 | |
Tris-base | VWR AMRESCO | 0497 | |
Boric acid | AMRESCO | 0588 | |
Urea | VWR AMRESCO | M123 | |
40% acrylamide/bisacrylamide (29:1) solution | BIO BASIC CANADA INC. | A0007 | |
Sucrose | Bioshop Canada inc. | SUC507 | |
Bromophenol blue | Bioshop Canada inc. | BRO777 | |
Xylenecyanol FF | SIGMA-ALDRICH | X-4126 | |
10% sodium dodecyl sulfate | Bioshop Canada inc. | SDS001 | |
Hydrochloric Acid (HCl) | CALEDON LABORATORIES LTD | 6026 | |
Sodium Chloride (NaCl) | Bioshop Canada inc. | SOD001 | |
4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) | Bioshop Canada inc. | HEP001 | |
Magnesium Chloride (II) hexahydrate | VWR AMRESCO | 0288 | |
Tween 20 | Bioshop Canada inc. | TW508 | |
Adenosine Triphospahte (ATP) | AMRESCO | 0220 | |
Sodium Acetate trihydrate (NaOAc) | SIGMA-ALDRICH | S8625 | |
Ethanol | Commercial Alcohols | P016EAAN | |
Tetramethyleneethylenediamine (TEMED) | AMRESCO | 0761 | |
10% Ammonium persulfate (APS) | BIO BASIC CANADA INC. | AB0072 | |
Succinimidyl 4-(N-maleimidomethyl) cyclohexane-1-carboxylate (SMCC) | ThermoFisher SCIENTIFIC | 22360 | |
Dimethyl sulfoxide (DMSO) | CALEDON LABORATORIES | 803540 | |
Urease | SIGMA-ALDRICH | U0251 | |
1x Phosphate Buffered Saline (PBS) | ThermoFisher SCIENTIFIC | 70011-069 | |
0.04% Phenol red | SIGMA-ALDRICH | P3532 | |
10x T4 polynucleotide kinase reaction buffer | Lucigen | 30061-1 | |
10x T4 DNA ligase reaction buffer | Bio Basics Canada | B1122-B | |
T4 DNA ligase (5 U/μl) | Thermo Fischer Scientific | B1122 | |
Luria Bertani (LB) Broth | AMRESCO | J106 | |
Agar | AMRESCO | J637 | |
T4 polynucleotide kinase (10 U/μl) | Lucigen | 30061-1 | |
E. coli K12 (MG1655) | ATCC | ATCC700926 | |
Centrifuge | Beckman Coulter, Inc. | 392187 | |
Glass plates | CBS scientific | ngp-250nr | |
0.75 mm thick spacers | CBS scientific | VGS-0725r | |
12-well comb | CBS scientific | VGC-7512 | |
UV Lamp | UVP | 95-0017-09 | |
Spectrophotometer (NanoVue) | GE Healthcare | N/A | |
Metal plate | CBS scientific | CPA165-250 | |
Vortex | VWR International | 58816-123 | |
Gel electrophoresis apparatus | CBS scientific | ASG-250 | |
Petri dishes | VWR International | 25384-342 | |
100 kDa MWCO centrifugal filters | EMD Millipore | UFC510024 | |
Magnetic Bead (BioMag) | Bangs Laboratories Inc | BM568 | |
Magnetic Seperation Rack | New England BioLabs | S1506S | |
Microfuge tubes | Sarstedt | 72.69 | |
Syringe filter (0.22 μm) | VWR International | 28145-501 | |
14 ml culture tube | VWR International | 60818-725 | |
Cell culture incubator | Eppendorf Scientific | M13520000 | |
Branson Ultrasonic cleaner | Branson | N/A | |
Camera (Canon Powershot G11) | Canon | N/A | |
50 ml conical tube | VWR International | 89004-364 |
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