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Kvantifisering Enkeltmicrovessel Permeabilitet i Isolert Blood-perfundert Rat Lung Forberedelse

Article DOI: 10.3791/51552-v • 07:22 min • June 30th, 2014
June 30th, 2014 •
1, 1,2
1Department of Physiology, The University of Tennessee Health Science Center, 2Department of Orthopedic Surgery and Biomedical Engineering, The University of Tennessee Health Science Center

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Det isolerte blod-perfusjon lunge preparatet gjør det mulig å visualisere microvessel nettverk på lungeoverflaten. Her beskriver vi en tilnærming til å kvantifisere permeabilitet av enkelt microvessels i isolerte lungene ved hjelp av sanntids fluorescens bildebehandling.

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Anatomi akutt lungeskade kapillærer, FITC dekstran fluorescens mikroskopi bildebehandling Metamorph venule
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