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Kvantifiera Single microvessel permeabilitet i Isolerad Blod-perfusion Rat Lung Förberedelse

Article DOI: 10.3791/51552-v • 07:22 min • June 30th, 2014
June 30th, 2014 •
1, 1,2
1Department of Physiology, The University of Tennessee Health Science Center, 2Department of Orthopedic Surgery and Biomedical Engineering, The University of Tennessee Health Science Center

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Den isolerade blod-perfusion lunga preparat gör det möjligt att visualisera mikrokärls nätverk på lungytan. Här beskriver vi en metod för att kvantifiera permeabilitet av enskilda mikrokärl i isolerade lungor med hjälp av realtids fluorescens avbildning.

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Anatomy akut lungskada kapillärerna, FITC dextran fluorescensmikroskopi bildbehandling metamorfa venule
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