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Quantificare singolo microvasi Permeabilità in Isolata Blood-perfusione Rat Lung Preparazione

Article DOI: 10.3791/51552-v • 07:22 min • June 30th, 2014
June 30th, 2014 •
1, 1,2
1Department of Physiology, The University of Tennessee Health Science Center, 2Department of Orthopedic Surgery and Biomedical Engineering, The University of Tennessee Health Science Center

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La preparazione isolato polmone sangue perfuso rende possibile visualizzare le reti microvasali sulla superficie polmonare. Qui si descrive un metodo per quantificare permeabilità delle singole microvasi nei polmoni isolati utilizzando l'imaging di fluorescenza in tempo reale.

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Anatomia umana danno polmonare acuto capillari, destrano FITC microscopia a fluorescenza immagini Metamorph venule
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