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Conformational Evaluation of HIV-1 Trimeric Envelope Glycoproteins Using a Cell-based ELISA Assay
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September 14, 2014
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Understanding viral surface antigens conformations is required to evaluate antibody neutralization and guide the design of effective vaccine immunogens. Here we describe a cell-based ELISA assay that allows the study of the recognition of trimeric HIV-1 Env expressed at the surface of transfected cells by specific anti-Env antibodies.
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Veillette, M., Coutu, M., Richard, J., Batraville, L., Désormeaux, A., Roger, M., Finzi, A. Conformational Evaluation of HIV-1 Trimeric Envelope Glycoproteins Using a Cell-based ELISA Assay. J. Vis. Exp. (91), e51995, doi:10.3791/51995 (2014).
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