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Ex Situ Normothermic Machine Perfusion of Donor Livers

Article DOI: 10.3791/52688-v • 12:13 min • May 26th, 2015
May 26th, 2015 •
1,2, 1,2, 1,2, 1,2, 1,2, 2, 1,2, 3, 4, 1,2
1Section of Hepato-Pancreato-Biliary Surgery and Liver Transplantation, University of Groningen, University Medical Center Groningen, 2Surgical Research Laboratory, Department of Surgery, University of Groningen, University Medical Center Groningen, 3Center of Engineering in Medicine/Surgical Services, Massachusetts General Hospital, Harvard Medical School, and Shriners Burns Hospital, 4Division of Transplantation, Department of Surgery, Massachusetts General Hospital, Harvard Medical School

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Here we present a protocol describing oxygenated ex situ machine perfusion of donor liver grafts. This article contains a step by step protocol to procure and prepare the liver graft for machine perfusion, prepare the perfusion fluid, prime the perfusion machine and perform oxygenated normothermic machine perfusion of the liver graft.

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Ex Situ Normothermic Machine Perfusion Donor Livers Static Cold Preservation Continuous Perfusion Organ Quality Viability Assessment Transplantation Protocol Human Donor Livers Device Hepatic Artery Portal Vein Perfusion Fluid Oxygenation Temperature Regulation Metabolic Activity Injury Assessment Biochemical Analysis Increase Number Of Suitable Livers For Transplantation
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