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TRAP-rc, Translating Ribosome Affinity Purification from Rare Cell Populations of Drosophila Embryos
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TRAP-rc, Translating Ribosome Affinity Purification from Rare Cell Populations of Drosophila Embryos

Article DOI: 10.3791/52985-v • 10:26 min • September 10th, 2015
September 10th, 2015 •
1, 1, 2, 1, 1
1University of Clermont-Ferrand, 2Memorial Sloan Kettering Cancer Center

章节

总结概括
Automatic Translation

Translating Ribosome Affinity Purification (TRAP) is able to capture cell-type-specific translation of mRNA. Here we report the first TRAP protocol dedicated to isolation of mRNA in rare cell populations of Drosophila embryos.

Tags

TRAP-rc Translating Ribosome Affinity Purification Rare Cell Populations Drosophila Embryos MRNA Translation Individual Cells Cellular Functions Proteins Point In Time Affinity Purification Polysomes Ribosomal Subunit Translatome Analyses Cell Types Developmental Processes Disease Development Progress Potential Therapies Molecular Level Optimized Version Gal4/UAS Targeting System Muscle Cells Global Gene Expression Analytics Microarrays RNA-seq Protocol Robustness Gal4 Drivers Cell-specific Genome-wide Studies
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