通过调整静电纺丝工艺为三层结构提供三种独特环境 体外 气道壁模型
13.2K 次观看
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被引用 17 次
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11:26 分钟
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2015年7月31日
DOI :10.3791/52986-v
2015年7月31日
•13.2K 次观看
Jack C. Bridge1 , Jonathan W. Aylott2 , Christopher E. Brightling5 , Amir M. Ghaemmaghami3 , Alan J. Knox4 , Mark P. Lewis6 , Felicity R.A.J. Rose1 , Gavin E. Morris1
1Division of Drug Delivery and Tissue Engineering, University of Nottingham, 2Laboratory of Biophysics and Surface Analysis, School of Pharmacy, University of Nottingham, 3Division of Immunology and Allergy, School of Molecular Medical Sciences, University of Nottingham, 4Division of Respiratory Medicine, School of Clinical Sciences, University of Nottingham, 5NIHR Respiratory Biomedical Research Unit, University of Leicester, 6School of Sport, Exercise, and Health Sciences, Loughborough University
生物材料技术的进步使得三维多细胞类型结构的构建成为可能。我们已开发出静电纺丝方案，用于制备三种独立的支架，以培养气道的主要结构细胞，从而构建气道细支气管壁的三维体外模型。
Chapters in this video
0:05
Title
1:49
Electrospinning Biphasic and Aligned Polyethylene Terephthalate Scaffolds
5:34
Sterilization of Scaffolds
6:24
Seeding of Fibroblasts and Epithelial Cells onto Biphasic Scaffolds and Airway Smooth Muscle onto Aligned Scaffolds
8:36
Tri-culture Using a Bioreactor System
9:29
Results: Tri-Culture Cells Continue to Produce Cell Type Specific Proteins
10:20
Conclusion
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