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Developmental Biology

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JoVE Journal Developmental Biology
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Analysis of Zebrafish Kidney Development with Time-lapse Imaging Using a Dissecting Microscope Equipped for Optical Sectioning

Article DOI: 10.3791/53921-v • 10:05 min • April 7th, 2016
April 7th, 2016 •
1, 1, 1,3, 1,2
1Molecular Genetics, Leibniz Institute on Aging - Fritz Lipmann Institute (FLI), 2Faculty of Biology and Pharmacy, Friedrich Schiller University of Jena, 3Carl Zeiss Microscopy GmbH

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The method described here allows time-lapse analysis of organ development in zebrafish embryos by using a fluorescence dissecting microscope capable of performing optical sectioning and simple strategies of readjustment to correct focal and planar drift.

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Zebrafish Kidney Development Time-lapse Imaging Dissecting Microscope Optical Sectioning Fluorescent Microscope Developmental Biology Living Embryos Time-lapsed Recording Sweeper Fish Embryo Larvae Early Developmental Processes Model Organisms Adult Organisms Transgenic GFP Expressing Line Plant Micro-injection
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