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Flat Mount Preparation for Observation and Analysis of Zebrafish Embryo Specimens Stained by Whole Mount In situ Hybridization
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Biology
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JoVE Journal Biology
Flat Mount Preparation for Observation and Analysis of Zebrafish Embryo Specimens Stained by Whole Mount In situ Hybridization

Flat Mount Preparation for Observation and Analysis of Zebrafish Embryo Specimens Stained by Whole Mount In situ Hybridization

DOI:
10.3791/51604-v

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06:36 min

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July 17, 2014

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, , , ,
1Department of Biological Sciences,University of Notre Dame

Chapters

  • 00:05Title
  • 01:37Dissection and Initial Deyolking of Embryo
  • 02:26Fine Deyolking of the Embryo
  • 03:18Mounting Embryo on a Glass Slide
  • 04:23Results: In situ Hybridization of Flat-mounted Embryos
  • 05:58Conclusion

Summary

Automatic Translation

Automatic Translation

The zebrafish embryo is an excellent model for developmental biology research. During embryogenesis, zebrafish develop with a yolk mass, which presents three-dimensional challenges for sample observation and analysis. This protocol describes how to create two-dimensional flat mount preparations of whole mount in situ (WISH) stained zebrafish embryo specimens.

Tags

Keywords: Zebrafish EmbryoDevelopmental StagesWhole Mount In Situ Hybridization (WISH)Flat Mount PreparationGene ExpressionEmbryonic StructuresDevelopmental BiologyBiomedical Research

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