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高通量基因定位在<em>布氏锥虫</em
High-throughput Gene Tagging in <em>Trypanosoma brucei</em>
JoVE 杂志
免疫与感染
This content is Free Access.
JoVE 杂志 免疫与感染
High-throughput Gene Tagging in Trypanosoma brucei

高通量基因定位在<em>布氏锥虫</em

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11:26 min

August 12, 2016

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11:26 min
August 12, 2016

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Summary

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Addition of a tag to a protein is a powerful way of gaining insight into its function. Here, we describe a protocol to endogenously tag hundreds of Trypanosoma brucei proteins in parallel such that genome scale tagging is achievable.

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