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An In Vitro Organ Culture Model of the Murine Intervertebral Disc

Article DOI: 10.3791/55437-v • 08:03 min • April 11th, 2017
April 11th, 2017 •
1,3, 2, 1, 2, 4, 3, 1,3,4
1Department of Biomedical Engineering, Washington University in St. Louis, 2Department of Biology, Washington University in St. Louis, 3Department of Orthopaedic Surgery, Washington University in St. Louis, 4Department of Materials Science and Mechanical Engineering, Washington University in St. Louis

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Whole organ culture of the intervertebral disc (IVD) preserves the native extracellular matrix, cell phenotypes, and cellular-matrix interactions. Here we describe an IVD culture system using mouse lumbar and caudal IVDs in their functional spinal units and several applications utilizing this system.

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In Vitro Organ Culture Model Murine Intervertebral Disc Inflammatory Response Homeostasis Intervertebral Disc Degeneration Low Back Pain New Drugs And Therapies Sterility Experimental Results Mouse Intervertebral Discs Dissection And Organ Culture Steps BALB/c Strain Mice Transgenic Animals Luciferase Gene Nuclear Factor Kappa B Promoter Euthanized Mouse Disinfecting The Skin And Coating Longitudinal Cut In Dorsal Skin Body Cavity Exposure Vertical Cuts In Spine
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