Journal
/
生物学
/
Techniques to Induce and Quantify Cellular Senescence
JoVE 杂志
生物学
需要订阅 JoVE 才能查看此.  登录或开始免费试用。
JoVE 杂志 生物学
Techniques to Induce and Quantify Cellular Senescence

Techniques to Induce and Quantify Cellular Senescence

DOI:
10.3791/55533-v

•

06:51 min

•

May 01, 2017

•

,
1Laboratory of Epidemiology and Population Science,National Institute on Aging, National Institutes of Health

Chapters

  • 00:05Title
  • 00:43DNA Damage-induced Senescence
  • 01:40Cell Staining for Senescence-associated (SA) Beta-Galactosidase
  • 03:30Quantifying SA Secretory Phenotype (SASP) Protein Levels
  • 04:37Results: Representative SA-beta-Galactosidase Staining and SASP Expression Analyses
  • 05:42Conclusion

Summary

自动翻译

自动翻译

Cellular senescence, the irreversible state of cell-cycle arrest, can be induced by various cellular stresses. Here, we describe protocols to induce cellular senescence and methods to assess markers of senescence.

Tags

Cellular SenescenceSenescence InductionSenescence QuantificationGamma IrradiationSenescence-associated Beta-galactosidase AssayCell CultureMicroscopyImage Analysis

Article

Embed

添加到播放列表

Usage Statistics

Related Videos

BioMEMS and Cellular Biology: Perspectives and Applications

Microinjection Techniques for Studying Mitosis in the Drosophila melanogaster Syncytial Embryo

Imaging Mismatch Repair and Cellular Responses to DNA Damage in Bacillus subtilis

Aseptic Laboratory Techniques: Plating Methods

RNAi Screening to Identify Postembryonic Phenotypes in C. elegans

Telomere Length and Telomerase Activity; A Yin and Yang of Cell Senescence

Advanced Confocal Microscopy Techniques to Study Protein-protein Interactions and Kinetics at DNA Lesions

Simple and Effective Administration and Visualization of Microparticles in the Circulatory System of Small Fishes Using Kidney Injection

Nasal Potential Difference to Quantify Trans-epithelial Ion Transport in Mice

Applications of Spatio-temporal Mapping and Particle Analysis Techniques to Quantify Intracellular Ca2+ Signaling In Situ

Read Article