Live Imaging of Primary Cerebral Cortex Cells Using a 2D Culture System

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被引用 2 次

10:12 分钟

2017年8月9日

10.3791/56063-v

2017年8月9日

7.9K 次观看

Live imaging is a powerful tool to study cellular behaviors in real time. Here, we describe a protocol for time-lapse video-microscopy of primary cerebral cortex cells that allows a detailed examination of the phases enacted during the lineage progression from primary neural stem cells to differentiated neurons and glia.

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Chapters in this video

0:05

Title

0:57

Dorsolateral Telencephalon Microdissection and Cell Plating

4:23

Time-lapse Video Microscopy

5:24

Cell Tracking

8:12

Results: Cell Lineage of Single Progenitor Cells

9:41

Conclusion

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