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Pattedyr celledeling i 3D matricer via kvantitative Konfokal refleksion mikroskopi

Article DOI: 10.3791/56364-v • 10:22 min • November 29th, 2017
November 29th, 2017 •
*1,2, *1, 1, 1, 3, 1,2, 3, 1,2,4
1Department of Chemical and Biomolecular Engineering, Johns Hopkins University, 2Johns Hopkins Physical Sciences - Oncology Center, Johns Hopkins University, 3Department of Biomedical Engineering, Johns Hopkins University, 4Departments of Oncology and Pathology and Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University School of Medicine
* These authors contributed equally

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Denne protokol effektivt undersøgelser pattedyr celledeling i 3D kollagen matricer ved at integrere synkronisering af celledeling, overvågning af division begivenheder i 3D matricer ved hjælp af live-celle imaging teknik, tidsopløst fluorescens refleksion mikroskopi og kvantitative imaging analyse.

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Bioteknologi sag 129 celledeling 3D Matrix synkronisering celle-Matrix interaktion Konfokal refleksion mikroskopi
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