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JoVE Journal Bioengineering
Däggdjur celldelning i 3D matriser via kvantitativa Confocal speglar mikroskopi
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Däggdjur celldelning i 3D matriser via kvantitativa Confocal speglar mikroskopi

Article DOI: 10.3791/56364-v • 10:22 min • November 29th, 2017
November 29th, 2017 •
*1,2, *1, 1, 1, 3, 1,2, 3, 1,2,4
1Department of Chemical and Biomolecular Engineering, Johns Hopkins University, 2Johns Hopkins Physical Sciences - Oncology Center, Johns Hopkins University, 3Department of Biomedical Engineering, Johns Hopkins University, 4Departments of Oncology and Pathology and Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University School of Medicine
* These authors contributed equally

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Detta protokoll studier effektivt däggdjur celldelning i 3D kollagen matriser genom att integrera synkronisering av celldelning, övervakning av division i 3D matriser använder live-cell bildteknik, tid-löst confocal speglar mikroskopi och kvantitativ bildanalys.

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Bioteknik frågan 129 celldelning 3D Matrix synkronisering Cell-Matrix interaktion Confocal speglar mikroskopi
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