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JoVE Journal Cancer Research
Radionuklidenes-fluorescens Reporter genet å spore svulst progresjon i gnager svulst modeller
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Radionuklidenes-fluorescens Reporter genet å spore svulst progresjon i gnager svulst modeller

Article DOI: 10.3791/57088-v • 10:04 min • March 13th, 2018
March 13th, 2018 •
1, 1, 1, 1, 1, 1, 1
1Department of Imaging Chemistry and Biology, School of Biomedical Engineering and Imaging Sciences, King's College London

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Vi beskriver en protokoll for prekliniske i vivo sporing av kreft metastasering. Den er basert på radionuklidenes-fluorescens reporter kombinere natrium iodide symporter, oppdaget av ikke-invasiv [18F] tetrafluoroborate-PET og en fluorescerende protein strømlinjeformet ex vivo bekreftelse. Metoden gjelder prekliniske i vivo celle sporing utover svulst biologi.

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Kreftforskning problemet 133 celle sporing reporter genet imaging natrium iodide symporter fluorescerende protein fantes et positron utslipp tomografi [18F] tetrafluoroborate fluorescens mikroskopi viral gen levering
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