一种简便有效的成人皮肤组织中人角质形成细胞的分离方法
10,077 Views
•
06:17 min
•
August 25, 2018
DOI:
Summary
Automatically generated
在这里, 我们提出了一个协议, 以有效地隔离主要的人角质细胞从成人皮肤组织。该方法利用接种培养基中的岩石抑制剂 Y-27632, 将表皮细胞与真皮细胞自发分离, 从而简化了常规程序。
Related Videos
08:49
A 3D Organotypic Melanoma Spheroid Skin Model
Related Videos
15671 Views
10:42
Generation and Culturing of Primary Human Keratinocytes from Adult Skin
Related Videos
16286 Views
10:03
Establishing a High Throughput Epidermal Spheroid Culture System to Model Keratinocyte Stem Cell Plasticity
Related Videos
3679 Views
08:26
Isolation and Culture of Adult Epithelial Stem Cells from Human Skin
Related Videos
28294 Views
07:39
Naïve Adult Stem Cells Isolation from Primary Human Fibroblast Cultures
Related Videos
14105 Views
N/A
Generating Primary Cultures for Keratinocyte Live Cell Imaging
Related Videos
122 Views
10:36
Isolation, Culture, and Characterization of Primary Schwann Cells, Keratinocytes, and Fibroblasts from Human Foreskin
Related Videos
6112 Views
10:51
Isolation and Culture of Primary Mouse Keratinocytes from Neonatal and Adult Mouse Skin
Related Videos
30834 Views
06:16
Isolation of Mouse Epidermal Keratinocytes and Their In Vitro Clonogenic Culture
Related Videos
17301 Views
06:28
Isolation and Culture of Primary Oral Keratinocytes from the Adult Mouse Palate
Related Videos
5826 Views
Read Article
Cite this Article
Liu, Z., Wen, J., Leng, X., Zhou, Q., Zhou, C., Zhao, H., Wu, X. A Simplified and Efficient Method to Isolate Primary Human Keratinocytes from Adult Skin Tissue. J. Vis. Exp. (138), e57784, doi:10.3791/57784 (2018).
Copy