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Journal / Immunology and Infection / Adskillelse af Rotte Epidermis og Dermis med…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Adskillelse af Rotte Epidermis og Dermis med termolysin til at opdage site-specifikke inflammatoriske mRNA og protein
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Adskillelse af Rotte Epidermis og Dermis med termolysin til at opdage site-specifikke inflammatoriske mRNA og protein

Article DOI: 10.3791/59708-v • 08:45 min • September 29th, 2021
September 29th, 2021 •
*1, *1, *1, 1, 2, 1
1Department of Anatomy and Cell Biology, Oklahoma State University Center for Health Sciences, 2Institute for Shock Physics, Washington State University
* These authors contributed equally

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Præsenteret her er en protokol for adskillelse af epidermis fra dermis at evaluere inflammatoriske mægler produktion. Efter betændelse adskilles rotte hindpote epidermis fra dermis ved termolysin ved 4 °C. Epidermis bruges derefter til mRNA-analyse af RT-PCR og proteinevaluering af vestlig blot og immunohistochemistry.

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Immunologi og infektion Problem 175 epidermis termolysin nervevækstfaktor interleukin-6 inflammation qPCR vestlig blotting immunohistochemistry
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