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Journal / Immunology and Infection / Karakterisering af inflammatoriske reaktioner…
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Immunology and Infection
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Karakterisering af inflammatoriske reaktioner under intranasal kolonisering med Streptococcus pneumoniae
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Karakterisering af inflammatoriske reaktioner under intranasal kolonisering med Streptococcus pneumoniae

Article DOI: 10.3791/50490-v • 09:12 min • January 17th, 2014
January 17th, 2014 •
1, 1, 1, 1
1Department of Pathology and Molecule Medicine, McMaster University

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Kolonisering af murine nasopharynx med Streptococcus pneumoniae og den efterfølgende ekstraktion af vedhængende eller rekrutterede celler er beskrevet. Denne teknik indebærer skylning af nasopharynx og indsamling af væsken gennem nares og kan tilpasses til forskellige udlæsninger, herunder differentialcellekvantificering og analyse af mRNA-udtryk in situ.

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Immunologi Streptococcus pneumoniae Nasal lavage nasopharynx murine flow cytometry RNA Kvantitative PCR rekrutterede makrofager neutrofiler T-celler effektorceller intranasal kolonisering
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