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Immunology and Infection

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JoVE Journal Immunology and Infection
Scheiding van rat epidermis en dermis met thermolysine om site-specifieke inflammatoire mRNA en eiwit te detecteren
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Scheiding van rat epidermis en dermis met thermolysine om site-specifieke inflammatoire mRNA en eiwit te detecteren

Article DOI: 10.3791/59708-v • 08:45 min • September 29th, 2021
September 29th, 2021 •
*1, *1, *1, 1, 2, 1
1Department of Anatomy and Cell Biology, Oklahoma State University Center for Health Sciences, 2Institute for Shock Physics, Washington State University
* These authors contributed equally

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Hier wordt een protocol gepresenteerd voor de scheiding van epidermis van dermis om de productie van ontstekingsmediators te evalueren. Na ontsteking wordt de epidermis van de achterpoot van de rat gescheiden door thermolysine bij 4 °C. De epidermis wordt vervolgens gebruikt voor mRNA-analyse door RT-PCR en eiwitevaluatie door westerse vlek en immunohistochistry.

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Immunologie en infectie Probleem 175 epidermis thermolysine zenuwgroeifactor interleukine-6 ontsteking qPCR westelijke blotting immunohistochistry
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