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Journal / Immunology and Infection / 高产细胞外囊泡制剂的纯化远离病毒
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Immunology and Infection

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高产细胞外囊泡制剂的纯化远离病毒

Article DOI: 10.3791/59876-v • 07:15 min • September 12th, 2019
September 12th, 2019 •
*1, *1, 1, 1, 1,2, 3, 4, 5, 1
1Laboratory of Molecular Virology, School of Systems Biology, George Mason University, 2American Type Culture Collection (ATCC), 3ATCC Cell Systems, 4Ceres Nanosciences, Inc, 5Department of Immunology and Nano-medicine, Herbert Wertheim College of Medicine, Florida International University
* These authors contributed equally

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该协议通过结合EV沉淀、密度梯度超离和颗粒捕获，将细胞外囊泡（EV）与高效和屈服的病毒分离，从而简化工作流程和减少启动体积要求，导致可重复的制剂用于所有EV研究。

Tags

免疫学和感染，第151期，细胞外囊，外泌体，病毒，纯化，密度梯度超离心，沉淀，EV富集纳米粒子
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