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Journal / Genetics / Cell Surface Reseptor identifikasjon ved hjelp av …
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Genetics

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JoVE Journal Genetics
Cell Surface Reseptor identifikasjon ved hjelp av genom-skala CRISPR / Cas9 genetiske skjermer
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CRISPR/Cas9 Editing of the C. elegans rbm-3.2 Gene using the dpy-10 Co-CRISPR Screening Marker and Assembled Ribonucleoprotein Complexes.
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Cell Surface Reseptor identifikasjon ved hjelp av genom-skala CRISPR / Cas9 genetiske skjermer

Article DOI: 10.3791/60803-v • 08:49 min • June 6th, 2020
June 6th, 2020 •
1,2, 1
1Cell Surface Signalling Laboratory, Wellcome Trust Sanger Institute, 2EMBL-EBI, Wellcome Genome Campus

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Dette manuskriptet beskriver en genom-skala celle-basert screening tilnærming for å identifisere ekstracellulære reseptor-ligand interaksjoner.

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Genetikk utgave 160 CRISPR/Cas9 rekombinantproteiner celleheft celleoverflatereseptor genomskalascreening ekstracellulære proteininteraksjoner
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