使用DNA进行细胞简单、经济且模块化图案化的技术
4.4K 次观看
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被引用 11 次
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08:59 分钟
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2021年2月24日
DOI :10.3791/61937-v
2021年2月24日
•4.4K 次观看
Katelyn A. Cabral1 , David M. Patterson2 , Olivia J. Scheideler1 , Russell Cole3 , Adam R. Abate4,5,6 , David V. Schaffer7,8 , Lydia L. Sohn9 , Zev J. Gartner2,6,10
1Graduate Program in Bioengineering, University of California San Francisco and University of California Berkeley, 2Department of Pharmaceutical Chemistry, University of California San Francisco, 3Scribe Biosciences, 4Department of Bioengineering and Therapeutic Sciences, University of California San Francisco, 5California Institute for Quantitative Biosciences, University of California San Francisco, 6Chan Zuckerberg Biohub, University of California San Francisco, 7Department of Chemical & Biomolecular Engineering, University of California Berkeley, 8Helen Wills Neuroscience Institute, University of California Berkeley, 9Department of Mechanical Engineering, University of California Berkeley, 10Center for Cellular Construction, University of California San Francisco
本文介绍一种利用DNA编程黏附技术在单细胞分辨率下对细胞进行微图案化的实验方案。该方案采用台式光刻平台在玻璃载片上构建DNA寡核苷酸的图案，随后使用 commercially available 互补寡核苷酸对细胞膜进行标记。寡核苷酸之间的杂交实现了程序化细胞黏附。
Chapters in this video
0:05
Introduction
0:30
Photopattern DNA onto Aldehyde-Functionalized Slides
2:46
Lift and Label Cells with Cholesterol-Modified DNA
4:21
Pattern the DNA-labeled Cells and Transferring into Hydrogel for 3D Culture
7:22
Results: Adhesion of CMO-Labeled Cells to DNA Patterns
8:32
Conclusion
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