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JoVE Journal Bioengineering
Une méthode de culture pour maintenir les cellules souches hématopoïétiques humaines quiescentes
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Une méthode de culture pour maintenir les cellules souches hématopoïétiques humaines quiescentes

Article DOI: 10.3791/61938-v • 07:14 min • May 17th, 2021
May 17th, 2021 •
1, 1
1Department of Stem Cell Biology, Research Institute, National Center for Global Health and Medicine

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Ce protocole permet le maintien des cellules souches hématopoïétiques humaines quiescentes in vitro en imitant le microenvironnement de moelle en utilisant des acides gras abondants, le cholestérol, des concentrations plus faibles de cytokines, et l’hypoxie.

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Bioingénierie Numéro 171 Cellules souches hématopoïétiques quiescent acides gras cholestérol hypoxie microenvironnement de moelle osseuse
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