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JoVE Journal Bioengineering
Um método cultural para manter células-tronco hematopoiéticas humanas quiescentes
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Um método cultural para manter células-tronco hematopoiéticas humanas quiescentes

Article DOI: 10.3791/61938-v • 07:14 min • May 17th, 2021
May 17th, 2021 •
1, 1
1Department of Stem Cell Biology, Research Institute, National Center for Global Health and Medicine

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Este protocolo permite a manutenção de células-tronco hematopoiéticas humanas quiescentes in vitro, imitando o microambiente de medula óssea usando ácidos graxos abundantes, colesterol, menores concentrações de citocinas e hipóxia.

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Bioengenharia Edição 171 Células-tronco Hematopoiéticas quiescente ácido graxo colesterol hipóxia microambiente de medula óssea
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