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Journal / Immunology and Infection / Overvågning af neutrofil elastase og cathepsin…
JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection
Overvågning af neutrofil elastase og cathepsin G-aktivitet i humane sputumprøver
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Overvågning af neutrofil elastase og cathepsin G-aktivitet i humane sputumprøver

Article DOI: 10.3791/62193-v • 09:23 min • May 21st, 2021
May 21st, 2021 •
*1,2, *1,2,3,4, 1,2,5,6,7, 1,3,8
1Translational Lung Research Center Heidelberg (TLRC), German Center for Lung Research (DZL), 2Dept. of Translational Pulmonology, University of Heidelberg, 3Molecular Medicine Partnership Unit (MMPU), European Molecular Biology Laboratory (EMBL), University of Heidelberg, 4Faculty of Biosciences, Collaboration for Joint Ph.D. Degree between EMBL and Heidelberg University, University of Heidelberg, 5Dept. of Pediatric Pulmonology, Immunology and Critical Care Medicine, Charité – Universitätsmedizin Berlin, 6Berlin Institute of Health (BIH), 7German Center for Lung Research (DZL), Associated Partner Site, Berlin, 8Dept. of Chemical Physiology and Biochemistry, Oregon Health and Science University
* These authors contributed equally

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De beskrevne protokoller indeholder en guide til at visualisere og kvantificere aktiviteten af neutrofile proteaser i menneskeligt spyt. Anvendelsen af en sådan analyse spænder fra evaluering af antiinflammatoriske behandlinger til biomarkørvalidering, lægemiddelscreening og store kliniske kohorteundersøgelser.

Tags

Immunologi og infektion Problem 171 Cystisk fibrose lungesygdom betændelse proteaser FRET-reportere neutrofil elastase cathepsin G diagnostik
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