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Journal / Immunology and Infection / Övervakning av Neutrophil Elastase och Cathepsin…
JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection
Övervakning av Neutrophil Elastase och Cathepsin G Aktivitet i humana Sputum-prover
Staphylococcus aureus Growth using Human Hemoglobin as an Iron Source
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Övervakning av Neutrophil Elastase och Cathepsin G Aktivitet i humana Sputum-prover

Article DOI: 10.3791/62193-v • 09:23 min • May 21st, 2021
May 21st, 2021 •
*1,2, *1,2,3,4, 1,2,5,6,7, 1,3,8
1Translational Lung Research Center Heidelberg (TLRC), German Center for Lung Research (DZL), 2Dept. of Translational Pulmonology, University of Heidelberg, 3Molecular Medicine Partnership Unit (MMPU), European Molecular Biology Laboratory (EMBL), University of Heidelberg, 4Faculty of Biosciences, Collaboration for Joint Ph.D. Degree between EMBL and Heidelberg University, University of Heidelberg, 5Dept. of Pediatric Pulmonology, Immunology and Critical Care Medicine, Charité – Universitätsmedizin Berlin, 6Berlin Institute of Health (BIH), 7German Center for Lung Research (DZL), Associated Partner Site, Berlin, 8Dept. of Chemical Physiology and Biochemistry, Oregon Health and Science University
* These authors contributed equally

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Protokollen häri beskrivs ge en guide för att visualisera och kvantifiera aktiviteten av neutrophil proteaser i mänskliga sputum. Tillämpningarna av sådan analys sträcker sig från utvärdering av antiinflammatoriska behandlingar, till biomarkörvalidering, läkemedelsscreening och stora kohort kliniska studier.

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Immunologi och infektion Utgåva 171 Cystisk fibros lungsjukdom inflammation proteaser FRET-reportrar neutrofila elastas cathepsin G diagnostik
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