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Journal / Immunology and Infection / Overvåking av Neutrophil Elastase og Cathepsin G…
JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection
Overvåking av Neutrophil Elastase og Cathepsin G Aktivitet i humane sputumprøver
An Advanced Murine Model for Nonalcoholic Steatohepatitis in Association with Type 2 Diabetes
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Rapid, Safe, and Simple Manual Bedside Nucleic Acid Extraction for the Detection of Virus in Whole Blood Samples
Journal (Immunology and Infection)
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Overvåking av Neutrophil Elastase og Cathepsin G Aktivitet i humane sputumprøver

Article DOI: 10.3791/62193-v • 09:23 min • May 21st, 2021
May 21st, 2021 •
*1,2, *1,2,3,4, 1,2,5,6,7, 1,3,8
1Translational Lung Research Center Heidelberg (TLRC), German Center for Lung Research (DZL), 2Dept. of Translational Pulmonology, University of Heidelberg, 3Molecular Medicine Partnership Unit (MMPU), European Molecular Biology Laboratory (EMBL), University of Heidelberg, 4Faculty of Biosciences, Collaboration for Joint Ph.D. Degree between EMBL and Heidelberg University, University of Heidelberg, 5Dept. of Pediatric Pulmonology, Immunology and Critical Care Medicine, Charité – Universitätsmedizin Berlin, 6Berlin Institute of Health (BIH), 7German Center for Lung Research (DZL), Associated Partner Site, Berlin, 8Dept. of Chemical Physiology and Biochemistry, Oregon Health and Science University
* These authors contributed equally

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Protokollene heri beskrevet gir en guide for å visualisere og kvantifisere aktiviteten til nøytrofile proteaser i menneskelig sputum. Anvendelsen av slik analyse spenner fra evaluering av antiinflammatoriske behandlinger, til biomarkørvalidering, legemiddelscreening og store kliniske kohortstudier.

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Immunologi og infeksjon utgave 171 cystisk fibrose lungesykdom betennelse proteaser FRET-reportere nøytrofil elastase cathepsin G diagnostikk
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