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Journal / Biochemistry / NMR-basert fragmentscreening i et minimumsutvalg, …
JoVE Journal
Biochemistry

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JoVE Journal Biochemistry
NMR-basert fragmentscreening i et minimumsutvalg, men maksimal automatiseringsmodus
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NMR-basert fragmentscreening i et minimumsutvalg, men maksimal automatiseringsmodus

Article DOI: 10.3791/62262-v • 09:19 min • June 4th, 2021
June 4th, 2021 •
*1, *1, 1,2, 1, 1, 1,2
1Institute for Organic Chemistry and Chemical Biology, Center for Biomolecular Magnetic Resonance (BMRZ), Johann Wolfgang Goethe-University Frankfurt, 2German Cancer Consortium (DKTK) and DKFZ
* These authors contributed equally

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Fragmentbasert screening av NMR er en robust metode for raskt å identifisere små molekylbindemidler til biomakromolekyler (DNA, RNA eller proteiner). Protokoller som beskriver automatiseringsbasert prøvepreparering, NMR-eksperimenter og innsamlingsbetingelser og analysearbeidsflyter presenteres. Teknikken muliggjør optimal utnyttelse av både 1H og 19F NMR-aktive kjerner for deteksjon.

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Denne måneden i JoVE utgave 172 Fragmentbasert screening Automatisering Løsning NMR-spektroskopi Datainnsamling og analyse Screeningplattform Fragmentbibliotek
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