Stabilized Longitudinal <em>In Vivo</em> Cellular-Level Visualization of the Pancreas in a Murine Model with a Pancreatic Intravital Imaging Window

Inwon Park; Pilhan Kim

doi:10.3791/62538

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Medicine

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Stabilized Longitudinal In Vivo Cellular-Level Visualization of the Pancreas in a Murine Model with a Pancreatic Intravital Imaging Window

稳定纵向在Vivo 细胞级可视化胰腺在穆林模型与胰腺内活性成像窗口

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06:52 min

May 6, 2021

DOI: 10.3791/62538-v

Inwon Park¹, Pilhan Kim^2,3

¹Department of Emergency Medicine,Seoul National University Bundang Hospital, ²Graduate School of Medical Science and Engineering,Korea Advanced Institute of Science and Technology, ³KI for Health Science and Technology,Korea Advanced Institute of Science and Technology

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胰腺的体内高分辨率成像利用胰腺内成像窗口获得便利。

此协议使用新的胰腺内成像窗口描述胰腺的稳定和重复的细胞水平在体内成像。这项技术的主要优点是，在活老鼠的胰腺中，在三周内可以进行一动不动的三维成像，分辨率达到细胞水平。首先准备手术平台，用70%乙醇对表面进行消毒。

麻醉后，使用带有自热控制加热垫的直肠探头来监测体温。从鼠标的左侧取下头发，然后用酒精和碘替代，从切口部位旋转到外表面，永不回到切口部位，以碘结束。然后，在老鼠的左侧做一个1.5厘米的切口，解剖皮肤和肌肉。

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