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Immunology and Infection

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JoVE Journal Immunology and Infection

Un sistema di coltura automatizzato da utilizzare nei test preclinici di terapie dirette all'ospite per la tubercolosi

Fluorescence-based Neuraminidase Inhibition Assay to Assess the Susceptibility of Influenza Viruses to The Neuraminidase Inhibitor Class of Antivirals

Journal (Immunology and Infection)

Fluorescence-based Neuraminidase Inhibition Assay to Assess the Susceptibility of Influenza Viruses to The Neuraminidase Inhibitor Class of Antivirals

Analysis of Fecal Microbiota Dynamics in Lupus-Prone Mice Using a Simple, Cost-Effective DNA Isolation Method

Journal (Immunology and Infection)

Analysis of Fecal Microbiota Dynamics in Lupus-Prone Mice Using a Simple, Cost-Effective DNA Isolation Method

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Un sistema di coltura automatizzato da utilizzare nei test preclinici di terapie dirette all'ospite per la tubercolosi

Article DOI: 10.3791/62838-v • 09:34 min • August 16th, 2021

August 16th, 2021 •

Seónadh O’Leary¹, Ahmad Z. Bahlool^1,2, Gemma O’Connor², Sally-Ann Cryan^2,3,4, Joseph M. Keane¹, Mary P. O’Sullivan¹

¹Department of Clinical Medicine, Trinity Translational Medicine Institute, St. James's Hospital, Trinity College Dublin, The University of Dublin, ²School of Pharmacy and Biomolecular Sciences, Royal College of Surgeons in Ireland (RCSI), ³SFI Advanced Materials and Bioengineering Research (AMBER) Centre, RCSI & TCD, ⁴SFI Centre for Research in Medical Devices (CURAM), RCSI, Dublin and National University of Ireland

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La quantificazione rapida ed efficiente della crescita intracellulare di M. tuberculosis è fondamentale per perseguire terapie migliori contro la tubercolosi (TB). Questo protocollo descrive un test di rilevamento colorimetrico basato sul brodo utilizzando un sistema di coltura liquida automatizzato per quantificare la crescita di Mtb nei macrofagi trattati con terapie candidate dirette dall'ospite.

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Immunologia e infezione numero 174

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