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Immunology and Infection

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JoVE Journal Immunology and Infection
Et automatisert kultursystem til bruk i preklinisk testing av vertsstyrte terapier for tuberkulose
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Et automatisert kultursystem til bruk i preklinisk testing av vertsstyrte terapier for tuberkulose

Article DOI: 10.3791/62838-v • 09:34 min • August 16th, 2021
August 16th, 2021 •
1, 1,2, 2, 2,3,4, 1, 1
1Department of Clinical Medicine, Trinity Translational Medicine Institute, St. James's Hospital, Trinity College Dublin, The University of Dublin, 2School of Pharmacy and Biomolecular Sciences, Royal College of Surgeons in Ireland (RCSI), 3SFI Advanced Materials and Bioengineering Research (AMBER) Centre, RCSI & TCD, 4SFI Centre for Research in Medical Devices (CURAM), RCSI, Dublin and National University of Ireland

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Rask og effektiv kvantifisering av intracellulær M. tuberculosis-vekst er avgjørende for å forfølge forbedrede terapier mot tuberkulose (TB). Denne protokollen beskriver en buljongbasert kolorimetrisk deteksjonsanalyse ved hjelp av et automatisert væskekultursystem for å kvantifisere Mtb-vekst i makrofager behandlet med kandidatvertsstyrte terapier.

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Immunologi og infeksjon utgave 174
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